WLP077 Tropicale yeast Blend - Thiol Releasing Non-GMO - PurePitch® Next Generation - BB: 18/03/2024

WLP077 Tropicale yeast Blend – Thiol Releasing Non-GMO – PurePitch® Next Generation – BB: 18/03/2024

£11.63

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Providing a balance of tropical flavors and aromas, such as passionfruit, grapefruit, and mango, that’s perfect for a juicy, hazy IPA!

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Description

This is a blend of non-GMO yeast strains that have been carefully selected to aid in the release of bound thiol compounds by targeting high enzymatic B-lyase activity. Providing a balance of tropical flavors and aromas, such as passionfruit, grapefruit, and mango, that’s perfect for a juicy, hazy IPA!

ATTENUATION :

75-82%

FLOCCULATION :

Low

ALCOHOL TOLERANCE :

Medium to High (8-12%)

FERMENTATION TEMPERATURE :

64° – 74° F

17° – 23° C

STA1 :

Negative

Suggested styles for WLP001 California Ale Yeast
  • American IPA
  • Double IPA
  • Hazy/Juicy IPA

We stock the largest range of yeast for home brewers in Europe, ensuring we have something for every beer style you could want to brew. Be sure to check it out here

Be sure to check out the White Labs Pitch rate calculator to ensure you have the right volume of yeast

Biotransformation

If you have been paying attention to the brewing world at all, you have heard the term “biotransformation”. Biotransformation refers to the interaction of yeast with hop compounds during fermentation. New research has emerged showing yeast to have the capacity to change various hop compounds into aromatic compounds that previously were not present, an approach that is becoming increasingly popular for juicy IPAs.

 

Yeast cells contain many types of enzymes that act as catalysts for a variety of biochemical reactions within the cell, often at varying activity levels. Some of these enzymes can trigger the conversion of bound hop compounds, resulting in unique aromas. Key enzymes identified in this biotransformation process include beta-glucosidase and beta-lyase.

 

Beta-glucosidase releases the aromatic terpenes from a non-aromatic glycoside precursor, such as geraniol from geranyl glycoside, while beta-lyase releases bound thiols (sulfur-based compounds) into free thiols (passionfruit and guava aromas).  Beta-lyase activity in yeast has lately been the focus of brewing research because of the resulting contribution of tropical characteristics such as passionfruit and guava.

Bound Thiols 

Free thiols, which are often responsible for contributing tropical aromas, are present in some of the most popular hops. However, many other hop varietals can contain higher amounts of odorless bound thiols. Yeast interactions from high B-lyase strains with these varieties of hops have been found to release these bound thiol compounds to free thiols resulting in new tropical aromas.

 

*The thiol concentrations in these less expensive hops can vary depending on growth conditions and season. The level of bound thiol present in the hops can directly be correlated with the levels of passionfruit and guava in a resulting beer.

IRC7

Beta-lyase enzymatic activity is strain dependent and can be traced back to the IRC7 gene. In collaboration with Oregon State University (Dr. Chris Curtin)  and Virgil Gamache Farms, Inc., White Labs set out to screen for beta-lyase activity in our strains.

 

The results showed varying degrees of beta-lyase activity in the strains that were analyzed. With this information, we wanted to validate the theory that high enzymatic activity would directly correlate to free thiols and, thus, the tropical characteristics in a finished beer.

Activity Assay

We first started by extracting the enzyme out of the yeast cell to measure activity levels (Cordente et al. 2019). They were compared with a known high activity control, Maxithiol, and known low activity control, S-288C. As seen in figure 1. Strains with higher than 1 relative activity were considered high-activity yeast.

Brewing Trial

We brewed with some of these strains to test our theory and determine if the sensory attributes in a beer would be perceivable. Using Cascade, Simcoe, and Mosaic for the whirlpool and dry hop additions, we conducted a split-batch fermentation using our new high-activity B-lyase blend, WLP077 Tropicale Yeast Blend, and WLP001 California Ale Yeast, a strain that had shown low enzyme activity in the assay. After fermentation, we sent these beers to be analyzed by Gas Chromatography for free thiol concentrations and a blind beer sensory panel was conducted.

 

Levels of Free thiols such as 3-sulfanylhexan-1-ol Acetate (3SHA), aromas of grapefruit and passionfruit, were present and above the threshold in our WLP077 Tropicale Yeast Blend.

Fig 2. Free Thiol 3SHA analyzed by GC. Flavor Threshold 5ng/L

The last step was correlating the analysis results with actual flavor differences in the beers by conducting a sensory test.

From the side-by-side tasting comparison, we collected and summarized the following tasting notes:

WLP001 – Resin, Dank, Pine

 

WLP077 – Passionfruit, Peach, Vanilla

 

Based on the results of our description test, the WLP001 California Ale Yeast was perceived to be similar to a West Coast IPA with resin and dank notes coming from the Cascade hops. The WLP077 Tropicale Yeast Blend was described as juicy, passionfruit, and vanilla, along with less lingering bitterness.

 

The results from our directional difference test (ASBC  Sensory-6) showcased a positive significant difference for a stronger intensity of passionfruit/grapefruit.

Which Sample Had the Stronger intensity of passionfruit/grapefruit?

 

WLP001 California Ale Yeast WLP077 Tropicale Yeast Blend
6 19

Level of Significance ≤ 0.05

Conclusion 

We sought to make a yeast blend that has not only high activity for biotransformation but also provides an all-natural blend that contributes to the balance of aroma and flavors known in Hazy IPAs.

Go out and experiment with this new blend and see what new flavors and aromas can be unlocked with hops!

Yeast Innovation and White Labs Brewing Co. 10th Anniversary 

The timing was perfect with the celebration of the 10th Anniversary of White Labs Brewing Co. for a brand new hazy IPA brew to debut in cans and showcase our newest strain innovation. White Labs Brewing Co. has been an integral part of our company, acting as a conduit for R&D to gain considerable information about our yeast strains, current and new, on a large brewing scale.  To mark 10 years of brewing innovation, we are bringing you this hazy IPA fermented with WLP090 San Diego Super Ale yeast and our NEW WLP077 Tropicale Yeast Blend.

Our goal in creating PurePitch® Next Generation for Homebrewers was to provide a product that will allow hobbyists and small scale brewers a convenient and modular package to pitch a consistent 7.5 million cells/mL; a commercially recommended pitching rate for standard ale ranges. The same package has proven to be an optimization to PurePitch® for larger professional brewers for over a year.

Now with more yeast, get a foolproof fermentation without a starter. Following a commercially recommended pitching rate allowing you to pitch like the pros!

We are the industry leader in quality with 38 quality checkpoints for every single strain. The same quality and care you trust, for both homebrew and professional yeast. You can be assured that all products released from White Labs have passed rigorous tests to ensure the highest viability and purity at the time of QC release.

With 79 Core strains and countless more in the Vault, we have has a strain for every beer, whether you’re using a trusty workhorse like WLP001 California Ale Yeast® or experimenting with something new and rare in the Vault.  Find these new strains at your local homebrew store carrying White Labs, or order direct from us!

Just twist the cap & pitch!

All of our people are passionate about what we do – a combination of Departments from Sales & Marketing, Customer Service, Microbiology, Production, Packaging, White Labs Brewing Co, White Labs Analytical Laboratory, Education, and R&D are dedicated to making the best yeast possible. It’s easy to love what you do in this industry which is why so many of our team members enjoy homebrewing, just as we did when we started in 1995. We encourage our teams to homebrew and build that love of brewing, the way it was when our company was founded.

PurePitch® Next Generation for Homebrewers FAQ

Is PurePitch® Next Generation larger than your previous package?

Yes!  Now with more yeast, you get double the pitch. PurePitch® Next Generation is designed to pitch at a rate of 7.5 million cells/mL per 5 gallons/20 liters, a commercially recommended pitching rate for standard ale ranges.

Do I need to make a starter?

No! For any standard ale, you will get the perfect fermentation by pitching directly – NO STARTER NEEDED.

What if I am brewing something that is not a “standard ale” – how do I know how much yeast I need for my batch?

Each pouch of PurePitch® Next Generation provides general quantity recommendations per 5gal/20L of wort.

​Our online calculator at yeastman.com allows you to see the exact recommendation for you using your volume, temperature, and gravity. Allowing you to customize the pitch rate to anything you want, larger or smaller!

What does 7.5 million cells/mL mean?

When pitching 1 pouch of PurePitch® Next Generation into 5 gal/20L of wort, there will be 7.5 million cells per milliliter.  This is a commercially recommended pitching rate, allowing you to pitch like the pros.

What is the shelf-life of PurePitch® Next Generation?

The shelf-life is 6 months from the QC-release date labeled on the package.  As a result of the proprietary dual-layer film, yeast are able to remain in excellent condition with high viability and performance.

Why change to a new version of PurePitch® ?

Our number one request from the homebrewing community has been for more yeast! Next Generation has been developed in response to brewing styles and fermentation techniques requiring more yeast than ever before, as well as the desire for a more optimal yeast package without the need for a starter.

Additional information

Weight 0.75 kg
Country of Origin US
HS Code 2102101090

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